British number one Johanna Konta lost to Venus Williams in the last 16 of the Italian Open in Rome.
The 26-year-old world number six recovered from a set down to level but American Williams, 36, dominated the final set to win 6-1 3-6 6-1.
Seven-time champion Rafael Nadal reached the last eight of the men’s event by beating Jack Sock 6-4 6-3.
Serbia’s Novak Djokovic is also through after defeating Spain’s Roberto Bautista-Agut 6-4 6-4.
However, world number three Stan Wawrinka suffered a 7-6 (7-1) 6-4 loss to big-serving American John Isner.