Five-time Wimbledon champion Venus Williams became the eighth of the top 10 seeds to be knocked out after a third-round defeat by Kiki Bertens.
Ninth seed Williams, 38, was beaten 6-2 6-7 (5-7) 8-6 by the 26-year-old Dutchwoman on Court One.
Williams’ fellow American Madison Keys lost nine consecutive games in her defeat to qualifier Evgeniya Rodina.
The 10th seed lost 7-5 5-7 6-4 in two hours 10 minutes against the Russian, who is ranked 120th in the world.
However, younger sister Serena went on to reach the last 16.